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Purpose The Facility functions to stimulate the competitiveness of biomolecular research activities at Oklahoma State University and throughout Oklahoma by providing access to essential advanced technologies, whose availability or price might otherwise be prohibitive to individual investigators. To fulfill this mission, the Facility performs specialized biochemical analyses, manages batteries of multi-user equipment, provides technical expertise, vends specialized biochemicals, and conducts hands-on workshops in the utilization of specific technologies. The Facility operates as a non-profit entity, providing services at or below actual costs. Major Services DNA Sequencing N-terminal Edman Protein/Peptide Sequencing MALDI-TOF mass spectrometry mass sizings Peptide mass fingerprint identifications (small-scale and high-throughput) de-novo sequencing
Robotic liquid handling High-throughput DNA isolation Vending of oligonucleotides, enzymes, kits, molecular standards, and reagents for DNA sequencing
Major multi-user equipment MALDI-TOF mass spectrometry [DE-PRO (Applied Biosystems) with reflector, CID module, and Proteomics Solution One, Mascot, Mascot Server, and Mascot Daemon workstations] Real-Time Polymerase Chain Reaction (Applied Biosystems 7500) Robotic solution handling Biomek 2000 Laboratory Automation Workstation (Beckman-Coulter) Symbiote MALDI plate spotter (Applied Biosystems) Digital imaging stations GelDoc 1000 System (BioRad) with LCD capture of UV and visible illumination, GS-525 Phosphorimager, GS-700 Densitiometer, laser- and thermal-printingAlphaInnotech HP Imager for routine fluorescent gel documentation, with thermal printing, file export, and quantitationAlphaInnotech HP Imager for high-sens, high res chemilluminescent applications, with printing, export, and quantiationTyphoon Trio fluorescence gel imager with DeCyder, ImageMaster 2D Platinum, and ImageQuant TL software applicationsNano-Calorimeter and BindWorks software (Calorimetry Sciences Corp.)
Minor multi-user equipment visible and fluorescent plate readers, UV/vis spectrophowtometer, DNA and protein speed vac's, gel dryer, hybridization incubator, vacuum oven, electroporation, thermal cyclers (7, including tube, plate, and gradient), DNA fluorometer, baking oven, Stratalinker, incubators, microfuges, shakers, misc. power supplies & electrophoresis apparati, X-ray exposure cassettes, biological computing workstations, et al. Training Workshops in Proteomics/Mass Spectrometry, et al.Individual consultations (DNA, RNA, & protein techniques)
Proposal pending with NSF: LTQ linear ion trap mass spectrometer with cLC
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Summary of Services 
